王小瑜,王相友,孙霞,孙冬霞.蔬菜有机磷农药残留检测试剂包检测条件的优化[J].农业机械学报,2008,39(4):97-100.
.[J].Transactions of the Chinese Society for Agricultural Machinery,2008,39(4):97-100.
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蔬菜有机磷农药残留检测试剂包检测条件的优化   [下载全文]
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DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  蔬菜  农药残留  乙酰胆碱酯酶  检测
基金项目:
王小瑜  王相友  孙霞  孙冬霞
山东理工大学
中文摘要:以商品化的乙酰胆碱酯酶试剂包为研究对象,比较了不同用量乙酰胆碱酯酶、底物和显色剂下的抑制率,并在此基础上进行了正交试验。在保证一定检测精度的前提下确定了酶用量为推荐用量的3/4,底物用量为推荐用量的3/4,显色剂用量为推荐用量的1/2,降低了检测成本。通过F检验证明,调整后配方和原配方之间无显著性差异。在此条件下,氧乐果、敌百虫和敌敌畏的检测限大约分别为0.37 mg/L、0.09 mg/L和0.035 mg/L,与原配方一致。
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Abstract:The optimization of determination for organophosphate pesticide residues in vegetables was studied. In the promise of higher sensitivity, a new low cost reagent components, whose dosage of acetylcholinesterase (AChE), substrate and chromogenic reagent were 3/4, 3/4 and 1/2 that of original components respectively, thus saved cost of reagent. The result was achieved through contrastive test. F-test proved that there was no significant difference between adjusted components and original components. The lower limit of determination of adjusted components to three pesticides is omethoate 0.37 mg/L, trichlorphon 0.09 mg/L, dichlorvos 0.035 mg/L, which is consistent with those of national standard rapid determination method.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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