徐红华,刘欣,温其标.复合酶法改善大豆分离蛋白乳化性的试验[J].农业机械学报,2008,39(2):103-106.
.[J].Transactions of the Chinese Society for Agricultural Machinery,2008,39(2):103-106.
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复合酶法改善大豆分离蛋白乳化性的试验   [下载全文]
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DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  大豆分离蛋白  复合酶  乳化性  优化设计
基金项目:
徐红华  刘欣  温其标
华南理工大学
中文摘要:运用配料试验设计解决了多酶复配的比例优化问题。采用米曲霉蛋白酶、木瓜蛋白酶、胰蛋白酶对大豆分离蛋白进行水解,建立复合酶配合比例与乳化性之间的数学模型。确定最佳比例为:米曲霉蛋白酶11.79%、胰蛋白酶32.93%、木瓜蛋白酶55.28%。最佳水解条件为:复合酶温度40℃、底物质量分数9%、酶添加量3%、pH值7.5、水解时间3h,乳化能力比原料提高了
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Abstract:50.86%。 An experimental method based on controlled mixture ratio of different enzymes in order to improve the emulsifying properties of soybean protein isolate was proposed. The model between the emulsifying properties of partial hydrolysis soybean protein and mixture ratio with aspergillus oryzae proteinase, papain and trypsin was established. The result of enzymes proportion was that aspergillus oryzae protease was 11.79%, papain was 32.93%, trypsin was 55.28%. The optimum hydrolysis condition was obtained by adding 3% of the multiple enzymes/substrate mass ratio to soybean protein isolate by way of dissolving at 9% in water and incubating 3?h. at 40℃ for pH7.5. The emulsifying capacity of hydrolyzing soybean protein with composite enzymes was increased up to 50.86% compared to the control sample.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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