磁场和抑制剂对莲藕多酚氧化酶反应动力学的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    通过添加Vc和外加磁场处理,研究莲藕多酚氧化酶(PPO)反应动力学的变化规律。以邻苯二酚为底物,莲藕PPO褐变反应动力学符合米氏方程所描述的单底物酶促反应动力学,Km为0.775mol/L,Vmax为11.150U/min。Vc对PPO有较强的抑制作用,反应动力学参数Km为0.081mol/L,Vmax为3.243U/min,呈现反竞争性抑制,表现为褐变出现滞后,随Vc浓度的增大滞后时间逐渐增长,且呈现S型趋势,并建立了相应的反应方程。不同磁场强度下,随着处理时间的变化,PPO活性呈现波动性。3.17A/m磁场强度下处理4h的酶液其反应动力学曲线表现为S型,并建立了相应的反应方程。

    Abstract:

    The change law of reaction dynamics was experimental studied through adding Vc to lotus root polyphenol-oxidase and dealing with lotus root polyphenol-oxidase (PPO) by magnetic field. The experimental results indicated that the reaction kinetic of enzymatic browning was accordant with Michaelis equation with catechol substrate, which Km and Vmax were 0.775 mol/L and 11.150U/min respectively. Vc had an inhibiting effect on lotus root PPO, which Km and Vmax were 0.081mol/L and 3.243U/min respectively. The browning was lagged and the lag time increased gradually with the rise in the concentration of the inhibitors, which was an anticompetitive inhibition. The PPO activity expressed a fluctuation at different magnetic field strengths and times. At 3.17A/m magnetic field strength for 4 hours, the reaction kinetics curve was a wave-type curve. The kinetic equations were established respectively.

    参考文献
    相似文献
    引证文献
引用本文

高梦祥,胡翠翠,严奉伟,张长峰.磁场和抑制剂对莲藕多酚氧化酶反应动力学的影响[J].农业机械学报,2008,39(1):78-81.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期: