Rotary transplanting mechanism is driven by noncircular gear train, and its structure is complicated, so it is very difficult to distinguish its motion interference through some common mechanism interference method. Based on kinematics model of rotary transplanting mechanism, the methods of distinguishing mechanism motion interference, which are the computer-human interactions and the faster distinguishing methods developed by the use of visual program of VB, are proposed respectively. The first method can make motion interference of mechanism visual, and motion interference can be distinguished visually and conveniently. Through the second method, motion interference can be distinguished automatically by program of VB. The above distinguishing methods provide an important theory platform for optimal design and parameter-design of the rotary transplanting mechanism.