黑木耳多糖提取工艺的优化
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    摘要:

    利用超微粉碎技术结合超声波协同纤维素酶提取黑木耳多糖,提高了黑木耳多糖的溶出率。建立了温度、pH值与黑木耳粗多糖溶出率的回归方程,获得了超声波协同纤维素酶酶解黑木耳工艺的最佳参数为:超声波频率20kHz、超声波功率20W、酶

    Abstract:

    解温度41.88℃、pH值4.61、时间160min。 The cell wall of Jew's ear is broken by the cellulase enzymatic hydrolysis with ultrasonic technology after processed by super micro-mill, which makes its polysaccharide goes through the broken cell into hydrolytic liquid, as a result, more Jew's ear polysaccharide will be gained. The parameters of the experiment were optimized by the experimental design method and a regress equation on temperature, pH and Jew's ear polysaccharide dissolving rate was developed by statistic analysis of the quadratic general rotary design. The optimized parameters of Jew's ear polysaccharide extraction by the cellulase enzymatic hydrolysis with ultrasonic technologies were obtained, which are ultrasonic frequency of 20kHz, ultrasonic power of 20W, enzymatic hydrolysis temperature of 41.88℃, pH4.61 and processing time of 160min.

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刘大纹,李铁柱,孙永海.黑木耳多糖提取工艺的优化[J].农业机械学报,2007,38(5):100-103.[J]. Transactions of the Chinese Society for Agricultural Machinery,2007,38(5):100-103

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