李良,周艳,滕飞,郭增旺,田甜,王中江.射流空化对大豆蛋白美拉德反应及产物乳化特性的影响[J].农业机械学报,2019,50(8):372-378.
LI Liang,ZHOU Yan,TENG Fei,GUO Zengwang,TIAN Tian,WANG Zhongjiang.Effects of Jet Cavitation on Maillard Reaction and Conjugate Structure and Emulsifying Properties of Soy Protein Isolate[J].Transactions of the Chinese Society for Agricultural Machinery,2019,50(8):372-378.
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射流空化对大豆蛋白美拉德反应及产物乳化特性的影响   [下载全文]
Effects of Jet Cavitation on Maillard Reaction and Conjugate Structure and Emulsifying Properties of Soy Protein Isolate   [Download Pdf][in English]
投稿时间:2018-12-16  
DOI:10.6041/j.issn.1000-1298.2019.08.041
中文关键词:  大豆分离蛋白  射流空化  美拉德反应  结构  乳化特性
基金项目:黑龙江省应用技术研究与开发技术重大项目(GA17B002)、中国博士后科学基金项目(2018M641798)、黑龙江省普通本科高等学校青年创新人才培养计划项目(NYPYSCT-2018163)、黑龙江省科学基金面上项目(C2018024)和国家重点研发计划项目(2016YFD0400402-2)
作者单位
李良 东北农业大学 
周艳 东北农业大学 
滕飞 东北农业大学 
郭增旺 东北农业大学 
田甜 东北农业大学 
王中江 东北农业大学 
中文摘要:为改善美拉德反应改性大豆分离蛋白效率低、反应时间长、能耗高等缺陷,研究了不同射流空化压力对大豆分离蛋白-葡聚糖美拉德反应进程的影响,并进一步研究射流空化压力对产物结构及乳化特性的影响。结果显示:当射流空化压力为1.5MPa时,SPI与葡聚糖美拉德反应进程最大,A420达到0.55,褐变程度提高了1702%,增加了中间产物含量(P<0.05),接枝度从32.54%增加到57.89%;SDS-PAGE验证了射流空化促进大豆分离蛋白-葡聚糖美拉德反应;射流空化处理后,SPI的荧光强度和紫外吸收峰升高,表明空化处理改变了蛋白分子空间,表面疏水性增强,但SPI-葡聚糖反应产物的荧光强度和紫外吸收峰降低,说明葡聚糖共价结合到处理后的SPI表面,其亲水基团增多,疏水性降低;SPI-葡聚糖美拉德反应产物的乳化活性、乳化稳定性分别提高了40.61%和48.46%。
LI Liang  ZHOU Yan  TENG Fei  GUO Zengwang  TIAN Tian  WANG Zhongjiang
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:soy protein isolate  jet cavitation  Maillard reaction  structure  emulsifying properties
Abstract:Maillard reaction is a non enzymatic, natural and non toxic method to modify protein or polysaccharide. The effect of jet cavitation powers on the process of soy protein isolate-dextran Maillard reaction was studied, to improve the defect of low efficiency, long reaction time and high energy consumption on the Maillard modified soy protein isolate. The effects of jet cavitation power on the products structure and emulsifying properties were further studied, which provided theoretical support for improving the functional properties of the soy protein isolate with Maillard reaction. The results showed that the jet cavitation treatment induced the molecular structure of the soy protein isolate and exposed more reactive sites, increased the activity of protein and promoted the Maillard reaction process. When the jet cavitation pressure was 1.5MPa, the SPI and dextran Maillard reaction process was the largest, A420 was 0.55, browning was increased by 17.02%, A249 was significant increased (P<0.05). The degree of graft was increased from 32.54% to 57.89%. SDS-PAGE verified that jet cavitation promoted the Maillard reaction of the soy protein isolate-dextran. The fluorescence intensity and UV absorption intensity of SPI was increased which was treated by jet cavitation, indicating that cavitation treatment changed protein conformation and increased the surface hydrophobicity, but the fluorescence and UV absorption intensity of SPI-dextran products were decreased, indicating that dextran combined with the treated SPI surface, the hydrophilic groups were increased and hydrophobicity was decreased. The emulsifying activity and emulsification stability of SPI-dextran Maillard reaction products were increased by 40.61% and 48.46%, respectively.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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