In order to achieve the combination of potted rice seedling transplanting and technology of mulchfilm, a kind of walktype potted rice seedling transplanting mechanism was designed. The process of seedling picking, conveying, film perforating and cooperating transplanting was completed by a set of rotary mechanisms. According to the working principle of the transplanting mechanism, kinematic theory model was set up and digital optimization goals were set. The computer aided analysis and optimization software of the transplanting mechanism based on Visual Basic 60 was developed. A set of parameters of the transplanting mechanism which met the requirements of transplanting were obtained by optimization. The twodimensional structure of the transplanting mechanism was designed. Threedimensional model was established. The virtual prototype simulation was completed by ADAMS software. The trajectory and attitude verification tests were carried out on the prototype using the test bench and highspeed photography technology. The results of bench test, simulation analysis and theoretical analysis were almost in agreement, which verified the rationality and correctness of the institutional design. The seedling pickup test, film perforating test and transplanting test were completed on the test bench. The results showed that the success rate of seedlings pickup was 90.4%. The success rate of transplanting for films of straw fiber and plastic was 87.5% and 82.5%, respectively, the results showed that the length and width of the film holes were good, which could meet the requirements of walktype potted rice seedling transplanting mechanism on film perforating part.