刘蕾,陈历水,马莺,高彦祥.EGCG对β-胡萝卜素乳液物理稳定性的影响[J].农业机械学报,2019,50(2):340-349.
LIU Lei,CHEN Lishui,MA Ying,GAO Yanxiang.Effect of EGCG on Physical Stability of β-carotene Encapsulated in Oil-in-water Emulsions[J].Transactions of the Chinese Society for Agricultural Machinery,2019,50(2):340-349.
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EGCG对β-胡萝卜素乳液物理稳定性的影响   [下载全文]
Effect of EGCG on Physical Stability of β-carotene Encapsulated in Oil-in-water Emulsions   [Download Pdf][in English]
投稿时间:2018-08-21  
DOI:10.6041/j.issn.1000-1298.2019.02.039
中文关键词:  β-胡萝卜素  乳状液  α-乳白蛋白  表没食子儿茶素没食子酸酯(EGCG)  稳定性
基金项目:国家自然科学基金面上项目(31371835)
作者单位
刘蕾 中粮营养健康研究院有限公司 
陈历水 中粮营养健康研究院有限公司 
马莺 哈尔滨工业大学 
高彦祥 中国农业大学 
中文摘要:以β-胡萝卜素为芯材,利用α-乳白蛋白(α-LA)为乳化剂制备了不同pH值的乳液,并利用表没食子儿茶素没食子酸酯(EGCG)作为抗氧化剂添加到乳液中,测定了乳液的粒径、电位、包埋率、快速稳定性、界面蛋白含量,考察了pH值和EGCG添加量对β-胡萝卜素乳液物理稳定性的影响,并对稳定性机理进行了研究。结果表明:EGCG添加量对pH值2.0和pH值7.0的乳液粒径、电位和包埋率没有显著影响(p>0.05),但对不同pH值乳液的快速稳定性影响显著(p<0.05)。在pH值2.0的乳液中,当EGCG添加量大于0.2%时,随着EGCG添加量的增加,乳液稳定性下降;在pH值7.0的乳液中,当EGCG添加量小于0.02%时,乳液的稳定性比未添加EGCG的乳液稳定性高;当EGCG添加量在0.02%~0.10%时,随着EGCG添加量的增大,乳液稳定性降低。当EGCG添加量大于0.10%时,EGCG的添加使β-胡萝卜素乳液快速分层,不能得到稳定乳液,原因是EGCG可以与α-LA相互作用使α-LA沉淀,致使没有足够的α-LA作为乳化剂稳定乳液。
LIU Lei  CHEN Lishui  MA Ying  GAO Yanxiang
COFCO Nutritionand Health Research Institution,COFCO Nutritionand Health Research Institution,Harbin Institute of Technology and China Agricultural University
Key Words:β-carotene  emulsion  α-lactalbumin  (-)-epigallocatechin-3-gallate  stability
Abstract:β-Carotene is used in food industry as a precursor of vitamin A with potent antioxidant activity through singlet oxygen quenching and deactivation of free radicals. Nevertheless, their utilization as nutraceutical ingredients in food industry is currently limited because of their poor water solubility, high melting point, chemical instability and low bioavailability. Emulsion based systems are often a better choice for delivering bioactive lipids such as β-carotene into functional foods since the emulsion increases bioavailability. The physical properties of β-carotene emulsions were characterized, which were stabilized by α-lactalbumin (α-LA) at different pH values with (-)-epigallocatechin-3-gallate (EGCG) as antioxidant. The particle size, zeta potential, encapsulation rate, stability index and interfacial protein of the emulsions were measured. EGCG addition had no effect on particle size, zeta potential and encapsulation rate of the emulsions, however, the addition of EGCG significantly influenced the physical stability of the emulsions. When the addition of EGCG was higher than 02% in the emulsions at pH value of 2.0, the physical stability was decreased with the increase of addition. The stability of emulsions at pH value of 7.0 was improved by the addition of EGCG in the range of 0.0025%~0.0200%, however, the stability of the emulsions was decreased when the EGCG addition was within 0.02%~0.10%. When the addition of EGCG was higher than 0.10%, the emulsions were separated into two phases quickly. In the mechanism study, the presence of EGCG changed the size and turbidity in α-LA solution when the addition was above 0.05%. Isothermal titration calorimetry (ITC) analysis implied that EGCG can interact with α-LA, with about five EGCG molecules integrated with one α-LA molecule. β-carotene emulsions destabilized by EGCG addition (greater than 0.10%) may due to the interaction between EGCG and α-LA.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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