徐红华,谢明明,丁瑞,马金玉,高子雯,关琛.CaCl2对纤维核诱导乳清浓缩蛋白聚合能力的影响[J].农业机械学报,2018,49(10):375-380.
XU Honghua,XIE Mingming,DING Rui,MA Jinyu,GAO Ziwen,GUAN Chen.Effect of CaCl2 on Aggregates of Whey Protein Concentrate with Adding Fibril Nuclei[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(10):375-380.
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CaCl2对纤维核诱导乳清浓缩蛋白聚合能力的影响   [下载全文]
Effect of CaCl2 on Aggregates of Whey Protein Concentrate with Adding Fibril Nuclei   [Download Pdf][in English]
投稿时间:2018-07-05  
DOI:10.6041/j.issn.1000-1298.2018.10.043
中文关键词:  乳清浓缩蛋白  纤维  CaCl2  均相核  二次核  聚合
基金项目:国家自然科学基金项目(31471682)
作者单位
徐红华 东北农业大学 
谢明明 东北农业大学 
丁瑞 东北农业大学 
马金玉 东北农业大学 
高子雯 东北农业大学 
关琛 东北农业大学 
中文摘要:研究了CaCl2对两种核诱导乳清浓缩蛋白(WPC)在聚合方面的影响。通过向WPC自发、均相核诱导WPC、二次核诱导WPC形成纤维过程中添加CaCl2,可提高纤维体系的硬度、黏度以及聚合率,并且对核诱导形成的纤维聚合物影响较大。添加CaCl2后,核诱导的硬度增加量是WPC自发硬度增加量的1.661倍(均相核诱导)和1.821倍(二次核诱导),黏度增加量的对应值为2.261倍(均相核诱导)与2.479倍(二次核诱导)。添加CaCl2使纤维的形态发生变化,成弯曲聚集体。对于纤维形成的两种路径自发与核诱导,CaCl2的影响程度不同,与乳清浓缩蛋白自发纤维聚合能力相比,CaCl2对核诱导乳清浓缩蛋白聚合能力的提升幅度更大。
XU Honghua  XIE Mingming  DING Rui  MA Jinyu  GAO Ziwen  GUAN Chen
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:whey protein concentrate  fibrils  CaCl2  homogeneous nuclei  secondary nuclei  aggregation
Abstract:Whey protein concentrate (WPC) fibril was made at pH value of 2.0 and 90℃. The investigation focused on the impact of adding CaCl2 on the aggregation of WPC spontaneous, homogeneous nuclei induced WPC and secondary nuclei induced WPC. As adding CaCl2, the properties of the hardness, viscosity and amount of WPC fibril aggregates with formation spontaneous or induced homogeneous/secondary nuclei were all increased, especially for two nuclei induced whey concentrate proteins. Adding CaCl2, the hardness was increased by 1.661 (homogeneous nuclei induced WPC) and 1.821 (secondary nuclei induced WPC) times than that of whey protein concentrate spontaneous, and the viscosity was increased by 2.261 (homogeneous nuclei induced WPC) and 2.479 (secondary nuclei induced WPC) times. The micro morphology using TEM of WPC aggregates was changed from long and semi-flexible fibrils into curly and cluster polymers when mixed CaCl2. And the fluorescence intensity was all decreased by adding CaCl2. It was speculated that because the Th T dye was unable to fully bind with the assembled fibrils. For the two ways of forming fibrils: spontaneous or nuclei induced WPC, there were different results that the aggregation ability of nuclei-induced WPC was even greater than that of spontaneous WPC fibril formation as adding CaCl2.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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