李 杨,李 红,齐宝坤,谢凤英,钟明明,檀 政.不同酶处理对生物解离乳状液结构及稳定性的影响[J].农业机械学报,2018,49(9):318-325.
LI Yang,LI Hong,QI Baokun,XIE Fengying,ZHONG Mingming,TAN Zheng.Effects of Different Enzyme Treatments on Structure and Stability of Proteins Extracted from Soybean Emulsion[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(9):318-325.
摘要点击次数: 79
全文下载次数: 87
不同酶处理对生物解离乳状液结构及稳定性的影响   [下载全文]
Effects of Different Enzyme Treatments on Structure and Stability of Proteins Extracted from Soybean Emulsion   [Download Pdf][in English]
投稿时间:2018-01-17  
DOI:10.6041/j.issn.1000-1298.2018.09.037
中文关键词:  大豆乳状液  生物酶  生物解离  稳定性
基金项目:国家重点研发计划项目(2016YFD0401402-01)和中国博士后科学基金面上项目(2017M612315)
作者单位
李 杨 东北农业大学 
李 红 东北农业大学 
齐宝坤 东北农业大学 
谢凤英 东北农业大学 
钟明明 东北农业大学 
檀 政 东北农业大学 
中文摘要:以生物解离技术提取的大豆乳状液为研究对象,探究不同生物酶处理(碱性蛋白酶Protex 6L、碱性蛋白酶Acalase 24L、溶血磷脂酶、磷脂酶A2和磷脂酶D)对乳状液的蛋白质结构和稳定性的影响。采用2种蛋白酶和3种磷脂酶对乳状液进行处理,通过对粒径分布、Zate电位、显微镜观察、红外光谱以及荧光光谱的测定可知:分层系数和游离油得率从大到小依次为溶血磷脂酶、碱性蛋白酶Acalase24L、碱性蛋白酶Protex 6L、磷脂酶D、磷脂酶A2;采用溶血磷脂酶酶解时,其电位值、平均粒径显著升高;通过显微镜观察到油滴聚集明显,这与电位值、粒径测定结果相一致;采用酶处理后乳状液的蛋白质二级结构中,α 螺旋相对含量均降低,无规卷曲相对含量均增加,其中,用溶血磷脂酶处理后的乳状液中α 螺旋相对含量最低,无规卷曲相对含量最高;所有样品经蛋白酶和磷脂酶酶解处理后,荧光强度均降低,在溶血磷脂酶酶解后乳状液中蛋白质的荧光强度最低;采用溶血磷脂酶、磷脂酶A2和磷脂酶D酶解乳状液后,PA含量均升高,PC和PE降低。
LI Yang  LI Hong  QI Baokun  XIE Fengying  ZHONG Mingming  TAN Zheng
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:soybean emulsion  enzyme  enzyme assisted  stability
Abstract:The enzyme assisted extracted soy emulsion was taken as object to explore the different biological enzyme treatments of emulsion protein structure and stability(Protex 6L, Acalase 24L〖JP〗 lysophospholipase, Phospholipase A2 and Phospholipase D). It was found that the emulsions were treated with two kinds of proteases and three phospholipases respectively, the particle size distribution, Zeta potential, microscopic observation, infrared spectrum and fluorescence spectrum were known, the results showed that the stratification coefficient and yield of free oil in decreasing trend were lysophospholipase, Acalase 24L, Protex 6L, phospholipase D, and phospholipase A2. Using lysophospholipase hydrolysis, the Zeta potential and the average particle size were increased significantly. The microstructure of the emulsion was found that the oil droplets were accumulated obviously, which was consistent with the results of potential and particle size. The protein secondary structure of the emulsion after the enzyme treatment, the α helix content was decreased and the random coil content was increased, and α helix content of the lowest, random coil content was the highest by using lysophospholipase. All samples by protease and phospholipase treatment, the fluorescence intensity was reduced, the fluorescence intensity of the protein was the lowest in the emulsion after lysophospholipase treatment. The content of PA was increased and the PC and PE were decreased by using lysophospholipase, phospholipase A2 and phospholipase D.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

   下载PDF阅读器