王 瑶,李 琪,李平兰.植物乳杆菌LPL-1产细菌素发酵培养基优化[J].农业机械学报,2018,49(9):311-317.
WANG Yao,LI Qi,LI Pinglan.Optimization of Fermentation Medium of Lactobacillus plantarum LPL-1 for Plantaricin LPL-1 Production by Response Surface Methodology[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(9):311-317.
摘要点击次数: 83
全文下载次数: 29
植物乳杆菌LPL-1产细菌素发酵培养基优化   [下载全文]
Optimization of Fermentation Medium of Lactobacillus plantarum LPL-1 for Plantaricin LPL-1 Production by Response Surface Methodology   [Download Pdf][in English]
投稿时间:2018-05-11  
DOI:10.6041/j.issn.1000-1298.2018.09.036
中文关键词:  植物乳杆菌LPL-1  植物乳杆菌素  发酵培养基  响应面
基金项目:国家自然科学基金面上项目(31671831)和公益性行业(农业)科研专项(201303014)
作者单位
王 瑶 中国农业大学 
李 琪 中国农业大学 
李平兰 中国农业大学 
中文摘要:乳酸细菌素是细胞在转录翻译过程中通过核糖体机制合成,并分泌到菌体体外的一类具有抑菌活性的蛋白质,细菌素具有无抗药性、无毒副作用及易被人体降解的特点,因此是一种天然的食品防腐保鲜剂。为了提高植物乳杆菌LPL-1所产细菌素的产量,以单增李斯特菌为指示菌,相对抑菌效价为效应值,通过响应面法对发酵培养基组成进行优化,确定了最优培养基组成。利用单因素试验与Plackett-Burman试验,确定了主要影响因素为葡萄糖质量分数、胰蛋白胨质量分数与吐温-80体积比,最陡爬坡试验与Box-Behnken响应面试验确定了最优培养基组成为:葡萄糖质量分数2.08%、酵母粉质量分数0.51%、胰蛋白胨质量分数1.02%、牛肉膏质量分数1%、吐温-80体积比1.02mL/L、磷酸氢二钾质量浓度3g/L、乙酸钠质量分数0.5%、硫酸镁质量浓度0.2g/L、硫酸锰质量浓度0.3g/L、柠檬酸氢二铵质量浓度2g/L、蒸馏水体积1L。在此条件下细菌素效价(752.11AU/mL)比优化前(286.67AU/mL)提高了1.62倍。因此,发酵培养基的优化为菌种与细菌素的产业化应用奠定了基础。
WANG Yao  LI Qi  LI Pinglan
China Agricultural University,China Agricultural University and China Agricultural University
Key Words:Lactobacillus plantarum LPL-1  plantaricin LPL-1  fermentation medium  response surface
Abstract:Bacteriocins from lactic acid bacteria (LAB) are ribosomally synthesized peptides or proteins that inhibit or kill the closely related species. Based on the merits of easy degradation by proteases, non resistance and non toxic side effects, plantaricin LPL-1 is a promising natural and safe biological preservative for food preservation industry. Aiming at the improvement of plantaricin LPL-1 from Lactobacillus plantarum LPL-1, the inhibitory activity (AU/mL) against Listeria monocytogenes 54002 was used as effect index, and the optimized medium composition was determined by response surface methodology. The major factors were determined as glucose, yeast extract and Tween-80 by single factor tests and Plackett-Burman tests. Based on the results of the steepest grade test and response surface methodology, the optimal medium composition was followed as glucose 208%, yeast extract 051%, tryptone 1.02%, beef extract 1%, Tween-80 1.02mL/L, K2HPO4 3g/L, NaAc 0.5%, MgSO4 0.2g/L, MnSO4 0.3g/L, tri ammonium citrate 2g/L, and ddH2O 1L. Under the optimal conditions, the inhibitory activity reached up to 75211AU/mL which was increased by 1.62 times. Finally, the research provided insight into the potential use of bacteriocin as a food preservative in the food industry.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

   下载PDF阅读器