王殿龙,艾 平,张衍林.稻秸酶解和沼气发酵臭氧氨水联合预处理技术研究[J].农业机械学报,2018,49(9):295-301.
WANG Dianlong,AI Ping,ZHANG Yanlin.Effect of Ozone and Soaking Aqueous Ammonia Pretreatment on Enzymatic Hydrolysis and Biogas Fermentation of Rice Straw[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(9):295-301.
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稻秸酶解和沼气发酵臭氧氨水联合预处理技术研究   [下载全文]
Effect of Ozone and Soaking Aqueous Ammonia Pretreatment on Enzymatic Hydrolysis and Biogas Fermentation of Rice Straw   [Download Pdf][in English]
投稿时间:2018-03-27  
DOI:10.6041/j.issn.1000-1298.2018.09.034
中文关键词:  稻秸  臭氧氨水联合预处理  酶解糖化  沼气发酵
基金项目:国家自然科学基金项目(51406064)
作者单位
王殿龙 淮阴工学院
江苏省生物质转化与过程集成重点实验室 
艾 平 华中农业大学 
张衍林 华中农业大学 
中文摘要:对稻秸进行了臭氧氨水联合预处理提高酶解糖化和沼气发酵效率的实验研究,探索了臭氧氨水联合预处理对木质纤维素降解、单糖质量浓度、还原糖质量浓度和沼气发酵气体产率的影响。结果表明:臭氧氨水两步预处理进一步强化了脱木质素能力,有效提高了稻秸酶解糖化效率和沼气发酵产气率,增强了纤维降解利用率。酶解过程中,〖JP2〗随着臭氧用量的增加,葡萄糖质量浓度先增加后降低,臭氧用量0.75g/g时葡萄糖质量浓度最高,为36.92g/L;〖JP〗氨水浸泡时间越长,还原糖质量浓度越高,氨水浸泡6h的还原糖质量浓度为60.51g/L。因此,酶解的适宜臭氧氨水联合处理条件为臭氧用量0.75g/g和氨水浸泡时间6h。对于沼气发酵,臭氧用量1.0g/g和氨水浸泡时间9h获得了较高甲烷产率,为165.39mL/g,有效提高了稻秸产气效率和木质纤维素利用率。
WANG Dianlong  AI Ping  ZHANG Yanlin
Huaiyin Institute of Technology;Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration,Huazhong Agricultural University and Huazhong Agricultural University
Key Words:rice straw  combined ozone and aqueous ammonia pretreatment  enzymatic hydrolysis  biogas fermentation
Abstract:The enzymatic hydrolysis and biogas fermentation of rice straw were studied. The effects of combined ozone and soaking aqueous ammonia pretreatment on the degradation of lignocellulose, mono sugars concentration, reducing sugars concentration and biogas production were explored. The results showed that combined ozone and aqueous ammonia pretreatment further increased the delignification ability, improved the saccharification efficiency of rice straw and biogas yield, and enhanced the fiber degradation efficiency. During enzymatic hydrolysis, the glucose concentration was increased first and then decreased with the increase of ozone dosage. When the ozone dosage was 0.75g/g, the glucose concentration was the highest, as 36.92g/L. The longer ammonia pretreatment time was, the higher the reducing sugar concentration was, and the ammonia concentration for 6h ammonia pretreatment was 60.51g/L. Therefore, the suitable combined pretreatment conditions for enzymatic hydrolysis was 0.75g/g ozone dosage and 6h of soaking aqueous ammonia. For biogas fermentation, a high methane yield of 165.39mL/g was obtained with 1.0g/g of ozone dosage and 9h of soaking aqueous ammonia time, which effectively increased the gas production efficiency of rice straw and utilization efficiency of lignocellulosic materials.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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