郭全友,修艳辉,王鲁民,姜朝军,王磊.鱼源莓实假单胞菌生长动力学与碳源利用分析[J].农业机械学报,2017,48(6):319-327.
GUO Quanyou,XIU Yanhui,WANG Lumin,JIANG Chaojun,WANG Lei.Growth Kinetics and Carbon Utilization Analysis of Pseudomonas fragi from Fish[J].Transactions of the Chinese Society for Agricultural Machinery,2017,48(6):319-327.
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鱼源莓实假单胞菌生长动力学与碳源利用分析   [下载全文]
Growth Kinetics and Carbon Utilization Analysis of Pseudomonas fragi from Fish   [Download Pdf][in English]
投稿时间:2016-10-25  
DOI:10.6041/j.issn.1000-1298.2017.06.042
中文关键词:  莓实假单胞菌  碳源利用能力  环境因子  生长动力学
基金项目:国家自然科学基金项目(31371867)、上海市自然科学基金项目(16ZR1444900)和中国水产科学研究院东海水产研究所基本科研业务费项目(2011M04、2014G02)
作者单位
郭全友 中国水产科学研究院东海水产研究所 
修艳辉 中国水产科学研究院东海水产研究所上海海洋大学食品学院 
王鲁民 中国水产科学研究院东海水产研究所 
姜朝军 中国水产科学研究院东海水产研究所 
王磊 中国水产科学研究院东海水产研究所 
中文摘要:以鱼源莓实假单胞菌为研究对象,利用BIOLOG获得15、25、33℃下其GEN III板中碳源生长数据,采用修正的Gompertz模型,结合孔平均颜色变化率及利用面积,分析其碳源利用效果,并对3个温度下pH值、NaCl和乳酸钠(NaL)的抑菌效应和生长动力学进行研究。结果表明,25℃时莓实假单胞菌总体碳源利用能力和活性最强,15℃时次之,33℃时最弱。在15~33℃内,碳源利用以糖类、羧酸类和氨基酸类为主。糖类中利用较好的有α-D-葡萄糖、L-岩藻糖等;氨基酸类中利用较好的有L-焦谷氨酸、L-丙氨酸、L-谷氨酸;羧酸类中利用较好的有奎宁酸、D-葡糖酸、糖质酸等。15~25℃下4%~8% NaCl及33℃下1%~8% NaCl对莓实假单胞菌皆有抑制作用。33℃时pH值对莓实假单胞菌生长起抑制作用,1%的NaL对其具有促进作用。通过对莓实假单胞菌抑菌效应和碳源利用分析,可为优化产品配方和保障产品质量提供理论依据。
GUO Quanyou  XIU Yanhui  WANG Lumin  JIANG Chaojun  WANG Lei
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;College of Food Science and Technology, Shanghai Ocean University,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences and East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
Key Words:Pseudomonas fragi  carbon utilization ability  environmental factors  growth kinetics
Abstract:Pseudomonas fragi is one of the specific spoilage organisms (SSOs) in aquatic food, especially in refrigeration stage. Aiming to investigate carbon utilization of P. fragi at different temperatures and study the effect of pH value, NaCl and sodium lactate on growth of P. fragi based on the kinetics of colour formation. Firstly, bacterial suspension was inoculated in Biolog GEN III plates, respectively incubating at different temperatures (15℃, 25℃ and 33℃). Then the data were fitted by the modified Gompertz equation and kinetic parameters were estimated. Through kinetic parameters (maximum specific growth rate and lag phase), and combining utilization area (S) and average well colour development (AWCD) value, the effect of carbon source utilization at different temperatures and environment factors on P. fragiwas analyzed. Results showed that at 25℃ P. fragihad the highest carbon utilization and activity ability, which was followed by 15℃, and the lowest value was got at 33℃. At 15~33℃, P. fragi mainly used carbohydrates, amino acids and carboxylic acids. Moreover, the effect of changes of different temperatures on various kinds of carbon sources utilization was obvious, while the effect of changes of different temperatures on utilization rates was not obvious. In carbohydrates α-D-glucose, L-fucose, sucrose and D-mannitol could be better utilizated. In amino acids L-pyroglutamic acid, D serine, L-alanine, L-glutamic acid could be better utilized. And in carboxylic acids quinic acid, D-gluconic acid, D-glucuronic acid, D-saccharic acid, L-malic acid were better utilized. At 15℃ the pH value was kept at 6.0 or 5.0, which the color maximum rates of color development were close, while at 25℃, pH value of 6.0 was far greater than at pH value of 5.0, and at 33℃ at any pH value, the growth of P. fragi was inhibited. At 15~25℃, 4%~8% NaCl and at 33℃, 1%~8% NaCl or pH value of 60 or pH value of 5.0, the growth of P. fragi was inhibited, and at 15~25℃, 1% NaCl or pH value of 6.0, pH value of 5.0 and at 33℃, 1% NaL the growth of P. fragi was promoted. The antibacterial effect on P. fragi and carbon utilization was analyzed which can provide a theoretical basis for optimizing product formulation and guaranteeing product quality.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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