Food safety issues caused by pesticide residues become the spotlight of public concerns all over the world. Metal-organic frameworks: porous coordination network 222 (MOFs:PCN 222) were synthesized and investigated as a novel sensitizer for AChE-inhibited method. TCPP (Tetrakis (4-carboxyphenyl)porphyrin) was employed as a ligand and highly stable Zr6 clusters were chosen as nodes for the assembly of stable Zr-MOFs. With carefully selected starting materials, a 3D porous MOFs was successfully constructed. Some properties of the prepared PCN 222, including structure, morphology X-ray diffraction (XRD) and thermostability were studied systematically. Porous virgate structure (spindle hexahedron), large surface area and strong thermostability were obtained for the MOFs. Based on the PCN 222, a sensitive AChE-inhibited method was developed. As a proof of concept, chlorpyrifos was used as a model analyte. Under the optimal conditions, absorbance increasing efficiency was increased by 68.8% (in 3min), which substantially improved the sensitivity of the colorimetric method compared with traditional ache inhibition method. The results revealed that the sensitization range obtained for chlorpyrifosis was approximately 0.01~10μg/mL. Furthermore, the biosensor was successfully applied to the quantitative assay of chlorpyrifos in synthetic sample (vegetable matrix). In addition, the method possessed the advantages of simplicity, rapidity and cost-effective. Hence, the developed sensitized AChE-inhibited method might provide a useful and practical tool for organophosphorus pesticide determination and related food safety analysis.