Aiming at the problem of unclear interactions between pre-cooling environmental factors and fruit quality in the post-harvest cold chain of perishable berries, the pre-cooling contribution rate evaluation indexes were proposed, and the impacts of delayed pre-cooling time (0h, 3h, 6h), ambient wind speed (0.1m/s, 0.5m/s, 1.0m/s, 1.5m/s), and ambient temperatures (0℃, 5℃, 10℃, 15℃) on the quality of the cold chain of blueberries were investigated, and a pre-cooled quality evaluation based on differential pressure pre-cooling was carried out. The quality of blueberries in the cold chain cycle was evaluated based on differential pressure pre-cooling. The results showed that immediate post-harvest pre-cooling could increase soluble solids content by 2.8 percentage points (pre-cooling contribution of 20.49%) and commercialisation rate by 6 percentage points (pre-cooling contribution of 6.74%), and the more timely pre-cooling was implemented, the more conducive it was to maintaining post-harvest quality; the improvement of ambient wind speed could enhance the effectiveness of pre-cooling, and the increase of pre-cooling wind speed up to 1.0m/s, the soluble solids content and commercialisation rate were increased by 1.75 percentage points and 4 percentage points, respectively (pre-cooling contribution of 15.15% and 4.34%); the pre-cooling treatment had a doublesided effect on the texture characteristics of fruits; in the pre-cooling stage, low ambient temperature and high wind speed would reduce the elasticity of fruits and increase the risk of transport damage, and in the storage and transport stage, the low ambient temperature and high wind speed were conducive to the maintenance of the elasticity and tightness of blueberries and improve the ability of the fruits to resist bumps. The optimal pre-cooling parameters were ambient temperature of 5℃ and ambient wind speed of 1.0m/s.