Based on the fact that existing seedlings pick-up test bench cannot meet the needs of various types of seedlings bowls and seedlings pick-up mechanisms for seedlings pick-up test, a kind of multipurpose seedlings pick-up test bench which was driven by multiple stepper motors was designed and it can adjust seedlings pick-up mechanisms inclination and program in the control system. It can adapt to different types of bowls and meet the test requirements of various types seedlings pick-up mechanisms by adjusting the position and posture of the mounting bracket, changing the distance between steel wire and the diameter of the circumference of the wire in the driving cage of the bowl. By editing the controller program, the linkage of three stepper motors can be controlled and the horizontal and longitudinal seedlings feed process of the seedlings feeding device can be matched with seedlings pick-up process of the seedlings pick-up mechanism. Modeling, assembling and virtual simulation of the test bench were carried out in 3D modeling software. Two types of seedlings bowls and three types of seedlings pick-up mechanisms were tested on the physical prototype produced by processing. The results of the three tests showed that the seedlings pick-up test could be successfully completed on the seedlings pick-up test bench, which proved that the test bench could meet the test needs of various types of seedlings bowls and seedlings pick-up mechanisms.