The physical and chemical characterization of biomass crushed straw (practical size from 5mm to 30mm) which from major grain producing areas was studied and analyzed according to the biomass standards and methods introduced here. The results showed physical and chemical characterization of corn straw from different areas has significant difference. For northeast area, the static angle of repose was maximum, the ash was minimum and bulk density was 17.25%, lower than that of other area. The physical and chemical characterization of different straw from the same area has significant difference, too. The dynamic external friction angle of corn and cotton straw was much higher than that of other crop straw, for bulk density, the soybean, cotton and peanut straw was maximum. The model of net calorific value and proximate analysis was developed. The verification results showed that the largest regression coefficient P value was less than 0.05.