Corn harvester is a large and complex agricultural machinery. The corn harvester is mainly used to complete the functions of picking, peeling, stalk returning, grain recovery and panicle collection. The connection and support way is complicated between vehicle frame and assembly components. In view of a domestic corn harvester, a set of finite element loading methods applied to the frame was proposed based on the connection mode and load arrangement. Then on the surface of the frame, eight measuring points were chosen to paste the strain gauge, and the frame was loaded by assembling the whole machine. Under two working conditions of no load and full load, the stress of each test point was tested. By comparing and analyzing the finite element calculations and test results, it could be found that the relative error of each points between the finite element analysis values and test values was within 20% except point 1, in addition, the finite element analysis values and test values were in good agreement. Thus, the feasibility of the finite element loading method of the corn harvester was verified. Furthermore, the finite element model could also be used to analyze and evaluate the reasonableness of the load distribution and structural design of the frame.