The pressure pulsation and flow-induced noise of outlet conduit have become two of important problems which have negative effect on axial-flow pumping system reliability. In order to investigate the flow-induced noise and pressure fluctuation characteristics of outlet conduit in axial-flow pumping system under different operating conditions, the fluctuating pressure and noised signals were obtained by pressure transduces mounted at different positions and a hydrophone in middle of outlet conduit. The experimental data were further analyzed by using short-time Fourier transform method at speeds of 1450 r/min, 1800 r/min and 2200 r/min. The results showed that with the increase of rotational speed, the root mean square of the pulsating amplitude of each measuring point was increased in the same flow rate ratio. With the increase of flow rate ratio, the root mean square of pulsating amplitude was decreased for each measuring point at the same rotational speed, while there were differences in the dominant frequency of each measuring point in different flow rate ratios. The latter was adjacent to 51Hz for the same measuring point at different flow rate ratios with different rotational speeds. The dominant frequency and subdominant frequency were not multiple relation with rotational frequency. The dominant frequency and subdominant frequency was in the range of 200Hz. With the increase of rotational speed, sound pressure level of flow-induced noise was increased in the same flow rate ratio. With the increase of flow rate, the sound pressure level of flow-induced noise was decreased firstly and then increased at the same rotational speed. The rotational speed had great influence on sound pressure level of flow-induced noise under the optimal condition. The research result had reference value for study on pressure pulsation and flow-induced noise in outlet conduit of pumping system.