4ZTL-2000 rice stripper combine harvester with air suction is a feasible machine for the harvesting of super rice. Deposition chamber is a key part of combine harvester. In order to obtain the outstanding performance and optimal parameters of the deposition chamber to clean super rice, quadratic orthogonal rotational combinational design was conducted. The factors were selected as inlet airflow velocity of the deposition chamber, mass and velocity of grain fed. Cleaning rate of grain and airflow pressure drop in the deposition chamber were objective indexes. The regression models between indexes and factors were developed by using Design-Expert software. The interactions of factors on the indexes were analyzed by the response surface method. The results show that inlet airflow velocity of the deposition chamber has the most significant effect on the indexes of the chamber, followed by velocity of grain fed, then mass of grain fed. In order to make the deposition chamber reach optimum performance to clean super rice under the mass of grain fed of 3.78kg/s in combine harvester, factors in the regression mathematical models were optimized to obtain the optimal parameters as inlet airflow velocity of deposition chamber of 13.93m/s, velocity of grain fed of 18.84m/s with cleaning rate of grain out of the deposition chamber of 90.26% and airflow pressure drop in the deposition chamber of 350.36Pa. The verified experimental results were that cleaning rate of grain out of the deposition chamber of 90.1% and airflow pressure drop in the deposition chamber of 3483Pa，which were consistent with the optimized results. The results offer the information to improve the performance of the deposition chamber to clean super rice in stripper combine harvester.