To improve battery energy efficiency and reduce power consumption of wireless collection nodes for greenhouse environment, hardware design for Greenhouse Wireless Acquisition nodes is achieved by low-power 16bit MCU (MSP430F149) and RF module CC1101. Power management module is designed based on chip TPS63031 to implement dual-power supply, which allows nodes’ power supplied respectively by three Nanfu AA batteries and one 3V button battery under working and sleeping mode. And 22.8% usable energy of batteries is improved effectively by using the DC-DC conversion. FSM module is adopted to software protocol design, implementing nodes’ task scheduling between working mode and sleeping mode. When the LPM3 mode is entered, node’s power consumption is greatly reduced. The discharge experiment of batteries and test of nodes performance show that design of power supply scheme in this paper guarantees node work for 110198h (about 4592d),with the setting of 30min acquisition cycle.