Efficient soil digging property of claw toe of Mus musculus was applied to the structure design of anti-drag subsoiler. The anti drag subsoiler had exponential curve shape of soil-cutting edge of shaft. To compare tillage resistance, the subsoiling experiments were conducted using bionic anti-drag subsoiler and traditional subsoiler. The results showed that both tillage depth and forward velocity could affect on subsoiling resistance significantly and the tillage resistance increased with the tillage depth and forward velocity. The tillage resistances of bionic anti-drag subsoiler were reduced by 8.5% to 39.5% respectively compared with traditional subsoiler under the same experimental conditions and the effect of reducing resistance was obvious.