The possibility of using two-stage ultrafiltration technique to separate ovalbumin and lysozyme from chicken egg white was investigated. Based on the mechanism of ultrafiltration, main factors that affecting the permeate flux and total proteins concentration in permeating liquor, such as initial feed concentration, pH value and transmembrane pressure (TMP) were studied. After ascertaining the ultrafiltration time by examining the change of permeate flux, the optimum ultrafiltration conditions were supposed to be as the follows: initial feed concentration, 6.0%; pH value, 2.5; TMP, 0.12MPa. In these optimum conditions, purity and yield of ovalbumin was 85.72% and 51.36% respectively, and that of lysozyme was 87.21% and 62.58% respectively, implying that separation efficiency was satisfied. The experimental results show that it is feasible for two-stage ultrafiltration technique to separate ovalbumin and lysozyme from chicken egg white in bulk and high efficiently.