A multi-channel vibration system for testing and analysis based on virtual instrument platform were established using the advanced graphical language LabVIEW. The header vibration signal (horizontal, vertical and axial direction) of Lovol combine harvester GN601—CR2Q at different sites under different conditions was measured by using the acceleration sensor. Time domain vibration and frequency domain were analyzed. The results of the header vibration test showed that the main excitation of the vibration sources was header transmission system, the horizontal vibration of header was largest and the largest header vibration position was the header conveyer. The frequency of inertia force of the cutter driving system was 10Hz. The frequency of inertia force of the engine was 30Hz. The natural frequency of the header was 68.8Hz.