Abstract:In order to determine the effectiveness of O3 on lignification of bamboo shoot (Phyllostachys pubescens Mazel), the bamboo shoots were exposed to either 100μL/L, 200μL/L or 300μL/L for 30min before stored at (1±1)℃ for 30d. The effect of O3 treatment on lignification and related enzyme activity were investigated. The results indicated that O3 treatment inhibited firmness from increasing, while decreasing the respiration rate and ethylene production. O3 treatment also retarded the activities of phenylalanine ammonia lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) increasing. The cellulose and lignin content were 8%~14% and 7%~12% lower than that of control, and the dacay rate was 71%~84% lower than that of control. Therefore, O3 treatment has potential use in delaying lignification of bamboo shoot.