23.7%。In order to enhance the alcohol yield of Saccharomyces cerevisiae DJ02, the preliminary fermentation conditions were optimized by single factor test. The results indicated that the fermentation conditions were: agar volume 80mL, inoculum concentration 11%, initial pH value 5.5, temperature 20℃, fermentation time 8d. The influence of twenty kinds of Chinese herbs, such as Pericarpium citri reticulatae, as promoting factors on the alcohol yield of Saccharomyces cerevisiae DJ02 was investigated. The influence of six kinds of Chinese herbs including Amomum villosum Lour on the alcohol yield of Saccharomyces cerevisiae DJ02 were significant. The optimum fermentation conditions were determined by plackettBurman design and response surface analysis. The optimum level of these fermentation conditions were: temperature 22℃, culture volume 75.0mL, pH value 5.71, fermentation time 8d, Amomum villosum Lour concentration 1.0%. The alcohol yield of Saccharomyces cerevisiae DJ02 was 16.92%, which was enhanced 23.7%. 