The method of data management and transmission for the mobile and intelligent agriculture information service system is studied, and the application layer protocol for wireless data transmission between the mobile terminal and the upper computer is designed based on the TCP/IP. The type, format, and content of the wireless data transmission are studied. The application layer protocol considers the collection and transmission characteristics of agricultural information. Initial applications indicate that the application layer protocol is suitable to the mobile and intelligent agriculture information service system, and has certain versatility and expansibility. The application layer protocol standardization is advantageous in sharing agricultural information.