With the aim to improve the purity of total flavonoids of Cyclocarya paliurus leaves, the suitable resin for separating and purifying total flavonoids of Cyclocarya paliurus leaves and the optimum parameters of purification were studied. The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests, and the separation techniques were evaluated by measuring the concentration of total flavonoids of Cyclocarya paliurus leaves with ultraviolet spectrophotometer. The results showed that the AB—8 macroporous resin gave the best effect of separation with the sampling rate 2BV/h and concentration 1.0mg/mL, respectively, and the concentration of elution was 70% alcohol, with eluting velocity 2BV/h. In conclusion, AB—8 resin could be applied to purify total flavonoids of Cyclocarya paliurus leaves.
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吴彩娥,方升佐,冯宗帅,杨万霞,李婷婷,贾韶千.青钱柳叶总黄酮大孔树脂纯化工[J].农业机械学报,2009,40(6):133-137. Process of Total Flavonoids in Cyclocarya paliurus Leaves with Macroporous Resins[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(6):133-137.