The commercial acetylcholinesterase (AChE) from Musca domestica, and the acetylcholinesterase made by the researchers themselves from fowl liver and cyprinoids brain, were used for pesticides detection. The inhibition of AChE activity under different concentrations of omethoate, trichlorfon and dichlorvos were compared. The results show that the inhibition of AChE activity of Musca domestica is the highest of the three above. Three kinds of Acetylcholinesterase are not sensitive to omethoate. AChE from cyprinoids brain can be used for rapid detection of dichlorvos in the case of high detection precision is not needed. AChE from fowl liver can be used for rapid detection of trichlorfon.
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王小瑜,孙霞,王相友,孙冬霞.不同来源酶快速检测有机磷农药的灵敏度比较[J].农业机械学报,2008,39(9):83-86.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(9):83-86.