董庆利,王海梅,Pradeep K Malakar,陆冉冉.气单胞菌在不同食物接触表面的存活与交叉污染[J].农业机械学报,2015,46(3):228-234.
Dong Qingli,Wang Haimei,Pradeep K Malakar,Lu Ranran.Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts[J].Transactions of the Chinese Society for Agricultural Machinery,2015,46(3):228-234.
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气单胞菌在不同食物接触表面的存活与交叉污染   [下载全文]
Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts   [Download Pdf][in English]
投稿时间:2014-10-31  
DOI:10.6041/j.issn.1000-1298.2015.03.033
中文关键词:  气单胞菌 擦拭取样法 恢复率 存活模型 交叉污染
基金项目:国家自然科学基金面上资助项目(31271896)、上海市自然科学基金面上资助项目(12ZR1420500)和上海市研究生创新基金资助项目(10-14-308-101)
作者单位
董庆利 上海理工大学 
王海梅 上海理工大学 
Pradeep K Malakar 食品研究所肠道健康与食品安全组 
陆冉冉 上海理工大学 
中文摘要:为探讨气单胞菌在不同食物接触表面的存活状况,采用擦拭取样法测定气单胞菌于案板、刀具表面的恢复率,并将气单胞菌分别接种于洁净和被污染的案板、刀具表面,评定气单胞菌在不同介质表面、不同时间下的存活状况。根据恢复率和介质表面上气单胞菌存活实测值,采用@Risk软件对介质表面实时带菌量的期望值进行计算,并运用一级线性模型对气单胞菌存活特征曲线进行拟合。同时,以案板为交叉污染途径,对气单胞菌在介质表面的存活行为对交叉污染的影响进行了探讨。结果表明:采用擦拭取样法测得的气单胞菌从案板、刀具表面的恢复率的最佳分布拟合结果分别是Normal(0.66,0.15)和Normal (0.72,0.18);气单胞菌在被污染的介质表面存活的时间(7h)长于其在洁净介质表面的存活时间(3h或2.5h),且一级线性模型可以较好地对气单胞菌存活曲线进行拟合(R2>0.88);蒙特卡罗模拟结果表明,忽略气单胞菌在食物接触表面的存活行为会高估其在厨房内的交叉污染水平。
Dong Qingli  Wang Haimei  Pradeep K Malakar  Lu Ranran
University of Shanghai for Science and Technology,University of Shanghai for Science and Technology,Gut Health and Food Safety, Institute of Food Research and University of Shanghai for Science and Technology
Key Words:Aeromonas spp. Swabbing method Recovery ratio Survival model Cross contamination
Abstract:The aim of present work is to study the cross contamination and survival capacity of Aeromonas spp. on different food contacts in the kitchen. The recovery ratios of Aeromonas spp. using swabbing method from cutting boards and knives were firstly examined. Then, the survival of Aeromonas spp. on clean and contaminated cutting boards, stainless steel knives surfaces was studied. Based on the survival data and recovery ratios of Aeromonas spp., expected numbers of Aeromonas spp. on cutting boards and knives were calculated. Mathematical models describing survival of Aeromonas spp. as a function of time were also proposed. Meanwhile, in term of cutting boards as the cross contamination approach, combining survival model and further study were conducted to investigate the influence of the survival of Aeromonas spp. on the cross contamination level. Results indicated that the best fitting of recovery ratios of Aeromonas spp. from cutting boards and knives obtained by @Risk were Normal (0.66, 0.15) and Normal (0.72, 0.18), respectively. And Aeromonas spp. proved to survive longer on the contaminated cutting boards and knives than on the clean ones. Survival data for Aeromonas spp. could be well described by linear model (R2>0.88), and models without survival models included would overestimate the risk associated to bacterial transfer.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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